The 6th Awaji International Forum on Infection and Immunity

Dennis John Grab, Ph.D.

Associate Professor
Department of Pediatrics
Division of Infectious Diseases
Johns Hopkins School of Medicine

Education and Training

A.B. 1969	Hunter College of City University of New York (Biology)
Ph.D. 1977	Cornell University Graduate School of Medical Sciences Sloan-Kettering Division (Pharmacology)
Postdoctoral Fellow 1977-1981
	Department of Cell Biology, The Rockefeller University

Professional Experience

Associate Professor
	Department of Pediatrics, Division of Infectious Diseases, Johns Hopkins School of Medicine, Baltimore, MD [September 2002-present]
Guest Associate Professor
	Department of Parasitology, Kurume University School of Medicine Fukuoka, Japan [January 2001-August 2002]
Assistant Professor
	Department of Pediatrics, Division of Infectious Diseases, Johns Hopkins School of Medicine, Baltimore, MD [January 2001- August 2002]
Associate Professor
	Department of Parasitology, Kurume University School of Medicine Fukuoka, Japan [April-December 2000]
Associate Professor
	Laboratory of Biochemistry Department of Applied Biological Sciences Saga University, Saga, Japan [1998- March 2000]
Assistant Professor
	Department of Tropical Medicine, Tulane University Medical Center, New Orleans, LA U.S.A. [1993-1997]
Associate Scientist
	Department of Parasitology, Tulane Regional Primate Research Center (TRPRC), Covington, LA 7073 U.S.A. [1993-1997]
Adjunct Assistant Professor
	Molecular Cell Biology Program, Tulane University New Orleans, LA U.S.A. [1993- 1997]
Visiting Scientist
	Division of Infectious Diseases, University of California at San Diego, San Diego, CA U.S.A. [1993]
Scientist
	Department of Biochemistry, International Laboratory for Research on Animal Diseases [ILRAD], Nairobi, Kenya [1983-1993]
Visiting Scientist
	Department of Biochemistry, International Laboratory for Research on Animal Diseases [ILRAD], Nairobi, Kenya [1981- 1982]
Postdoctoral Research Fellow
	Department of Cell Biology, The Rockefeller University, New York, NY U.S.A. [1977-1980] Sponsor: Dr. Philip Siekevitz
Predoctoral Research Fellow
	Sloan-Kettering Institute For Cancer Research, New York, NY U.S.A. [1970-1977] Sponsors: Dr. Morris Zedeck, Dr. Mary Petermann, Dr.Mary Hamilton
Part-time Laboratory Technician
	Department of Clinical Microbiology, The New York Hospital, New York, NY. U.S.A. [1975-1977]
Senior Research Technician
	Dr. Colvin Redman, The New York Blood Center, New York, NY. U.S.A. [1969-1970]

Recent publications (recent 5 years)

1.	Nikolskaia, O.V., Kim, Y.V., Kovbasnjuk, O., Kim, K.J., Grab, D.J. \Entry of Trypanosoma brucei gambiense into microvascular endothelial cells of the human blood-brain barrier. For Int. J. Parasitol. Thematic isssue Int. J. Parasitol. 36:513-519, 2006.
2.	Nyarko, E.K., Grab, D.J., Dumler, J.S. Anaplasma phygocytophilum-infected neutrophils enhance transmigration of Borrelia burgdorferi across the human blood-brain barrier. For Thematic isssue Int. J. Parasitol. 36:601-605, 2006.
3.	Dumler, J.S., Choi, K,S,, Garcia-Garcia, J.C., NBarat, N,S, Scorpio, D.G., Garyu, J.W., Grab, D.J., Bakken. J.S. Human Granulocytic Anaplasmosis and Anaplasma phagocytophilum. Emerging Inf. Dis. 11 (12):1828-1834, 2005
4.	Grab, D.J., Perides, G., Dumler, J.S., Kim, K.J., Park, J., Kim, YV., Nikolskaia, O, Choi, K.S., Stins, M.F., Kim, K.S. Borrelia burgdorferi traversal of an in vitro human blood-brain barrier model requires host-derived proteases. Infect. Immun. 73(2):1014-22, 2005.
5.	Justin W.A. Garyu, J.W.A., Choi , K.S., Grab, D.J., Dumler, J.S., Defective phagocytosis in Anaplasma phagocytophilum-infected neutrophils. Infection. Immun. 73(2):1187-90, 2005.
6.	Grab, D.J., Nikolskaia, O., Kim, Y.V., Lonsdale-Eccles, J.D., SIto, S., Hara, T., Fukuma, T., Nyarko, E., Kim, K.J., Stins, M., Delannoy, M.J., Rodgers, J., Kim, K.S. African Trypanosome Interactions with an in vitro model of the Human Blood-Brain Barrier. J. Parasitology 90: 970-979, 2004. Note: Figure 7b made the cover of October issue.
7.	Nyarko E., Hara, T., Grab, D.J., Kim, Y.V., Nikolskaia, O., Fukuma, T., Tabata, M. In vitro toxicity of Pd(II) and Au(III) porphyrins and their aqueous metal ion counterparts on Trypanosoma brucei brucei growth. Chem. Biol. Interactions 148: 19-25, 2004.
8.	Park, J., Grab, D.J., J. S. Dumler. Anaplasma phagocytophilum AnkA binds to granulocyte DNA and nuclear proteins. Cell. Microbiol. 6: 743-51, 2004.
9.	Choi, K.S., Grab, D.J., Dumler, J.S. Anaplasma phagocytophilum infection induces protracted neutrophil degranulation. Infect. Immun.72: 3680-3683, 2004.
10.	Grab, D.J., Salem, M.L., Dumler, J.S., Bucala, R. A Role for the Peripheral Blood Fibrocyte in Leishmania? Trends Parasitol. 20: 12, 2004.
11.	Kuboki, N., Inoue, N., Sakurai, T., Di Cello, F., Grab, D.J., Suzuki, H., Sugimoto, C., Igarashi, I. Loop-mediated Isothermal Amplification (LAMP) and its application for the detection African Trypanosomes. J. Clin. Microbiol. 4: 5517-5524, 2003.
12.	Park, J., Choi, K.S., Scorpio, D., Grab, D.J., Dumler, J.S. Divergent interactions of Ehrlichia chaffeensis and Anaplasma phagocytophilum infected leukocytes with endothelial cell barriers. Infect. Immun. 71: 6728-6733, 2003.
13.	Park, J., Kim, K.J., Grab, D.J., Dumler, J. S. Anaplasma phagocytophilum major surface protein-2 (Msp2) forms multimeric complexes in the bacterial membrane. FEMS Microbiol. Letters. 227: 243-247. 2003.
14.	Grab, D.J., Salem, M., Chesney, J., Bucala, R., Lanners, H-N. A role for peripheral blood fibrocytes in Lyme disease? Medical Hypotheses 59: 1-10, 2002.
15.	Lonsdale-Eccles, Grab, D.J. Trypanosome hydrolases and the Blood-brain barrier. Trends Parasitol., 18: 17-19. 2002.
16.	Nyarko,E., Hara, T., Grab, D.J., Tabata, M., Fukuma, T. Toxic Effects of Mercury(II), Cadmium(II) and Lead(II) Porphyrins on Trypanosoma brucei brucei growth. Chem. Biol. Interactions, 139: 177-185, 2002.
17.	Grab, D.J., Lonsdale-Eccles, J.D., Oli, M.W., Corbeil, L.B. Lactoferrin binding proteins of Tritrichomonas foetus. J. Parasitology 87: 1064-1070, 2001.
18.	Naessens, J., Grab, D.J., Sileghem, M. Identifying the mechanisms of trypanotolerance in cattle. In: S.J. Black and J.R. Seed Eds, World Class Parasites, Vol. 1, The African Trypanosomes, pp 97-112. Kluwer Academic Publishers, 2001. ISBN 0-7923-7512-2.
19.	Sileghem, M., Saya, R., Grab, D.J., Naessens, J. Induction of TNF by the variable surface glycoprotein from African trypanosomes: accessory role of the diacylglycerol moiety of the gycosylphosphatidylinositol anchor in stimulation of bovine monocytes. Vet. Immunol. Immunopathol. 78: 325-339, 2001.